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KMID : 1134819990280030670
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Journal of the Korean Society of Food Science and Nutrition
1999 Volume.28 No. 3 p.670 ~ p.676
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Effects of Exercise and / or High Fat Diet on Carnitine and Carnitine Palmitoyltransfersase - I mRNA Levels in Rats
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Sohn Hee-Sook
Oh Suk-Heung
Cha Youn-Soo
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Abstract
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The effect of exercise and/or high-fat diet on carnitine status and carnitine palmitoyltransferase-I(CPT-I) level were investigated in Weanling Sprague-Dawley rats. The rats were fed an AIN-76 diet or a modified high-fat AIN diet, supplemented with 35% corn oil, for 31 days. During the 31-day period half of the animals in each dietary group were exercised on a treadmill for 90 minutes per day. Carnitine concentrations were determined in plasma and liver and CPT-I mRNA levels were measured by Northern blot analysis with CPT-I cDNA probe in livers of rats. Exercise rats gained less weight than non-exercised rats during the study for high fat diet group. Exercise rats had a higher plasma acid-soluble acylcarnitine and acid insoluble acylcarnitine concnetrations than non-exercised rats for normal diet group. Exercise or high fat diet increased liver carnitine concentration, but a mixed effect was not shown. In exercised rats, CPT-I mRNA levels increased significantly relative to those of nonexercised rats. CPT-I mRNA levels also increased when compared high-fat fed rats with those of normal diet fed rats. These data suggest that there is a correlation between carnitine concentrations and CPT-I mRNA levels and that CPT-I can be regulated at the transcriptional level by exercise and/or high fat diet.
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KEYWORD
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exercise, high-fat, carnitine, CPT-I
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